a3 vector Search Results


plasmid expression vectors coding for a 3×κb-directed luciferase reporter  (Promega)
90
Promega plasmid expression vectors coding for a 3×κb-directed luciferase reporter
Plasmid Expression Vectors Coding For A 3×κb Directed Luciferase Reporter, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid expression vectors coding for a 3×κb-directed luciferase reporter/product/Promega
Average 90 stars, based on 1 article reviews
plasmid expression vectors coding for a 3×κb-directed luciferase reporter - by Bioz Stars, 2026-06
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stim1 encoding sequence  (Promega)
90
Promega stim1 encoding sequence
Functional analysis of the N-FLAG-tagged Orai1 channel. A, thapsigargin (TG)-induced [Ca2+]i rises in Orai1-expressing HEK293 cells. Left, average time course of Ca2+ responses induced by 2 μm TG in Orai1 (gray triangles)-, N-FLAG-tagged Orai1 (black circles)-, and control vector (white circles)-transfected HEK293 cells (n = 27–28). The perfusion solution was first changed to 0.5 mm EGTA containing Ca2+-free HBS (EGTA), and 2 μm TG was applied to the cells in the absence of extracellular Ca2+. Eleven minutes after the application of TG, 2 mm Ca2+ was added to the extracellular solution. Right, maximum [Ca2+]i rises (Δ[Ca2+]i) induced by TG in EGTA and those after re-addition of 2 mm external Ca2+. B, TG-induced [Ca2+]i rises in Orai1 and <t>STIM1-coexpressing</t> HEK293 cells. Left, average time course of Ca2+ responses induced by 2 μm TG in Orai1 plus STIM1 (gray triangles)- and N-FLAG-tagged Orai1 plus STIM1 (black circles)-cotransfected HEK293 cells and control vector (white circles)-transfected HEK293 cells (n = 25–41). Right, Δ[Ca2+]i induced by TG in EGTA and those after re-addition of 2 mm external Ca2+. C, activation of CRAC current induced by 40 μm IP3 in Orai1- and STIM1-coexpressing cells. Left, representative current-voltage relationships in Orai1 plus STIM1 (a)- and N-FLAG-tagged Orai1 plus STIM1 (b)-cotransfected HEK293 cells and control vector (c)-transfected HEK293 cells. Data represent leak-subtracted current densities (pA/picofarad) evoked by 50-ms voltage ramps from -100 to +100 mV. Right, average CRAC current densities at -80 mV in Orai1 plus STIM1 (n = 11), N-FLAG-tagged Orai1 plus STIM1 (n = 9) and control (n = 9) cells. **, p < 0.01; and ***, p < 0.001.
Stim1 Encoding Sequence, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stim1 encoding sequence/product/Promega
Average 90 stars, based on 1 article reviews
stim1 encoding sequence - by Bioz Stars, 2026-06
90/100 stars
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pkh97, an expression vector containing a 3.0-kb cdna insert of hil-5r b chain  (Schering-Plough corporation)
90
Schering-Plough corporation pkh97, an expression vector containing a 3.0-kb cdna insert of hil-5r b chain
Functional analysis of the N-FLAG-tagged Orai1 channel. A, thapsigargin (TG)-induced [Ca2+]i rises in Orai1-expressing HEK293 cells. Left, average time course of Ca2+ responses induced by 2 μm TG in Orai1 (gray triangles)-, N-FLAG-tagged Orai1 (black circles)-, and control vector (white circles)-transfected HEK293 cells (n = 27–28). The perfusion solution was first changed to 0.5 mm EGTA containing Ca2+-free HBS (EGTA), and 2 μm TG was applied to the cells in the absence of extracellular Ca2+. Eleven minutes after the application of TG, 2 mm Ca2+ was added to the extracellular solution. Right, maximum [Ca2+]i rises (Δ[Ca2+]i) induced by TG in EGTA and those after re-addition of 2 mm external Ca2+. B, TG-induced [Ca2+]i rises in Orai1 and <t>STIM1-coexpressing</t> HEK293 cells. Left, average time course of Ca2+ responses induced by 2 μm TG in Orai1 plus STIM1 (gray triangles)- and N-FLAG-tagged Orai1 plus STIM1 (black circles)-cotransfected HEK293 cells and control vector (white circles)-transfected HEK293 cells (n = 25–41). Right, Δ[Ca2+]i induced by TG in EGTA and those after re-addition of 2 mm external Ca2+. C, activation of CRAC current induced by 40 μm IP3 in Orai1- and STIM1-coexpressing cells. Left, representative current-voltage relationships in Orai1 plus STIM1 (a)- and N-FLAG-tagged Orai1 plus STIM1 (b)-cotransfected HEK293 cells and control vector (c)-transfected HEK293 cells. Data represent leak-subtracted current densities (pA/picofarad) evoked by 50-ms voltage ramps from -100 to +100 mV. Right, average CRAC current densities at -80 mV in Orai1 plus STIM1 (n = 11), N-FLAG-tagged Orai1 plus STIM1 (n = 9) and control (n = 9) cells. **, p < 0.01; and ***, p < 0.001.
Pkh97, An Expression Vector Containing A 3.0 Kb Cdna Insert Of Hil 5r B Chain, supplied by Schering-Plough corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pkh97, an expression vector containing a 3.0-kb cdna insert of hil-5r b chain/product/Schering-Plough corporation
Average 90 stars, based on 1 article reviews
pkh97, an expression vector containing a 3.0-kb cdna insert of hil-5r b chain - by Bioz Stars, 2026-06
90/100 stars
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pgem4z-mage-a3-64a vector  (Argos Therapeutics)
90
Argos Therapeutics pgem4z-mage-a3-64a vector
Functional analysis of the N-FLAG-tagged Orai1 channel. A, thapsigargin (TG)-induced [Ca2+]i rises in Orai1-expressing HEK293 cells. Left, average time course of Ca2+ responses induced by 2 μm TG in Orai1 (gray triangles)-, N-FLAG-tagged Orai1 (black circles)-, and control vector (white circles)-transfected HEK293 cells (n = 27–28). The perfusion solution was first changed to 0.5 mm EGTA containing Ca2+-free HBS (EGTA), and 2 μm TG was applied to the cells in the absence of extracellular Ca2+. Eleven minutes after the application of TG, 2 mm Ca2+ was added to the extracellular solution. Right, maximum [Ca2+]i rises (Δ[Ca2+]i) induced by TG in EGTA and those after re-addition of 2 mm external Ca2+. B, TG-induced [Ca2+]i rises in Orai1 and <t>STIM1-coexpressing</t> HEK293 cells. Left, average time course of Ca2+ responses induced by 2 μm TG in Orai1 plus STIM1 (gray triangles)- and N-FLAG-tagged Orai1 plus STIM1 (black circles)-cotransfected HEK293 cells and control vector (white circles)-transfected HEK293 cells (n = 25–41). Right, Δ[Ca2+]i induced by TG in EGTA and those after re-addition of 2 mm external Ca2+. C, activation of CRAC current induced by 40 μm IP3 in Orai1- and STIM1-coexpressing cells. Left, representative current-voltage relationships in Orai1 plus STIM1 (a)- and N-FLAG-tagged Orai1 plus STIM1 (b)-cotransfected HEK293 cells and control vector (c)-transfected HEK293 cells. Data represent leak-subtracted current densities (pA/picofarad) evoked by 50-ms voltage ramps from -100 to +100 mV. Right, average CRAC current densities at -80 mV in Orai1 plus STIM1 (n = 11), N-FLAG-tagged Orai1 plus STIM1 (n = 9) and control (n = 9) cells. **, p < 0.01; and ***, p < 0.001.
Pgem4z Mage A3 64a Vector, supplied by Argos Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgem4z-mage-a3-64a vector/product/Argos Therapeutics
Average 90 stars, based on 1 article reviews
pgem4z-mage-a3-64a vector - by Bioz Stars, 2026-06
90/100 stars
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human ephrin a3 gene orf cdna clone in cloning vector  (Sino Biological)
N/A
Full length Clone DNA of Human sapiens ephrin A3
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human adenosine a3 receptor adora3 transcript variant 2 gene orf cdna clone in cloning vector  (Sino Biological)
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Full length Clone DNA of Human adenosine A3 receptor transcript variant 2
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Image Search Results


Functional analysis of the N-FLAG-tagged Orai1 channel. A, thapsigargin (TG)-induced [Ca2+]i rises in Orai1-expressing HEK293 cells. Left, average time course of Ca2+ responses induced by 2 μm TG in Orai1 (gray triangles)-, N-FLAG-tagged Orai1 (black circles)-, and control vector (white circles)-transfected HEK293 cells (n = 27–28). The perfusion solution was first changed to 0.5 mm EGTA containing Ca2+-free HBS (EGTA), and 2 μm TG was applied to the cells in the absence of extracellular Ca2+. Eleven minutes after the application of TG, 2 mm Ca2+ was added to the extracellular solution. Right, maximum [Ca2+]i rises (Δ[Ca2+]i) induced by TG in EGTA and those after re-addition of 2 mm external Ca2+. B, TG-induced [Ca2+]i rises in Orai1 and STIM1-coexpressing HEK293 cells. Left, average time course of Ca2+ responses induced by 2 μm TG in Orai1 plus STIM1 (gray triangles)- and N-FLAG-tagged Orai1 plus STIM1 (black circles)-cotransfected HEK293 cells and control vector (white circles)-transfected HEK293 cells (n = 25–41). Right, Δ[Ca2+]i induced by TG in EGTA and those after re-addition of 2 mm external Ca2+. C, activation of CRAC current induced by 40 μm IP3 in Orai1- and STIM1-coexpressing cells. Left, representative current-voltage relationships in Orai1 plus STIM1 (a)- and N-FLAG-tagged Orai1 plus STIM1 (b)-cotransfected HEK293 cells and control vector (c)-transfected HEK293 cells. Data represent leak-subtracted current densities (pA/picofarad) evoked by 50-ms voltage ramps from -100 to +100 mV. Right, average CRAC current densities at -80 mV in Orai1 plus STIM1 (n = 11), N-FLAG-tagged Orai1 plus STIM1 (n = 9) and control (n = 9) cells. **, p < 0.01; and ***, p < 0.001.

Journal:

Article Title: Tetrameric Orai1 Is a Teardrop-shaped Molecule with a Long, Tapered Cytoplasmic Domain *

doi: 10.1074/jbc.M900812200

Figure Lengend Snippet: Functional analysis of the N-FLAG-tagged Orai1 channel. A, thapsigargin (TG)-induced [Ca2+]i rises in Orai1-expressing HEK293 cells. Left, average time course of Ca2+ responses induced by 2 μm TG in Orai1 (gray triangles)-, N-FLAG-tagged Orai1 (black circles)-, and control vector (white circles)-transfected HEK293 cells (n = 27–28). The perfusion solution was first changed to 0.5 mm EGTA containing Ca2+-free HBS (EGTA), and 2 μm TG was applied to the cells in the absence of extracellular Ca2+. Eleven minutes after the application of TG, 2 mm Ca2+ was added to the extracellular solution. Right, maximum [Ca2+]i rises (Δ[Ca2+]i) induced by TG in EGTA and those after re-addition of 2 mm external Ca2+. B, TG-induced [Ca2+]i rises in Orai1 and STIM1-coexpressing HEK293 cells. Left, average time course of Ca2+ responses induced by 2 μm TG in Orai1 plus STIM1 (gray triangles)- and N-FLAG-tagged Orai1 plus STIM1 (black circles)-cotransfected HEK293 cells and control vector (white circles)-transfected HEK293 cells (n = 25–41). Right, Δ[Ca2+]i induced by TG in EGTA and those after re-addition of 2 mm external Ca2+. C, activation of CRAC current induced by 40 μm IP3 in Orai1- and STIM1-coexpressing cells. Left, representative current-voltage relationships in Orai1 plus STIM1 (a)- and N-FLAG-tagged Orai1 plus STIM1 (b)-cotransfected HEK293 cells and control vector (c)-transfected HEK293 cells. Data represent leak-subtracted current densities (pA/picofarad) evoked by 50-ms voltage ramps from -100 to +100 mV. Right, average CRAC current densities at -80 mV in Orai1 plus STIM1 (n = 11), N-FLAG-tagged Orai1 plus STIM1 (n = 9) and control (n = 9) cells. **, p < 0.01; and ***, p < 0.001.

Article Snippet: For functional analysis, Orai1 encoding sequence was also subcloned into pCI-neo (Promega, Madison, WI), and mouse STIM1 encoding sequence was subcloned from pApuro-FLAG-tagged mouse STIM1 ( 31 ) into pCI-neo.

Techniques: Functional Assay, Expressing, Control, Plasmid Preparation, Transfection, Activation Assay

Predicted topology of Orai1 and a docking model with STIM1. A, schematic diagram of N-FLAG Orai1. Orai1 is a highly glycosylated protein with glycan moiety of ∼8 kDa per subunit. The Orai1 polypeptide has a cytosolic N terminus, four TM segments (yellow columns: S1–S4), and a cytosolic C terminus with a putative coiled-coil sequence (green box) involved in binding with STIM1. The FLAG tag (red box) was introduced in the N terminus. B, illustration of putative docking between Orai1 and STIM1. The three-dimensional reconstruction in this study suggests that the cytoplasmic domain of Orai1 is long enough for direct association with STIM1. STIM1 contains a coiled-coil motif (green box) in the cytoplasm, a single TM segment (yellow column), a sterile α motif domain (purple box), and an EF-hand motif (blue crescent) in the ER lumen.

Journal:

Article Title: Tetrameric Orai1 Is a Teardrop-shaped Molecule with a Long, Tapered Cytoplasmic Domain *

doi: 10.1074/jbc.M900812200

Figure Lengend Snippet: Predicted topology of Orai1 and a docking model with STIM1. A, schematic diagram of N-FLAG Orai1. Orai1 is a highly glycosylated protein with glycan moiety of ∼8 kDa per subunit. The Orai1 polypeptide has a cytosolic N terminus, four TM segments (yellow columns: S1–S4), and a cytosolic C terminus with a putative coiled-coil sequence (green box) involved in binding with STIM1. The FLAG tag (red box) was introduced in the N terminus. B, illustration of putative docking between Orai1 and STIM1. The three-dimensional reconstruction in this study suggests that the cytoplasmic domain of Orai1 is long enough for direct association with STIM1. STIM1 contains a coiled-coil motif (green box) in the cytoplasm, a single TM segment (yellow column), a sterile α motif domain (purple box), and an EF-hand motif (blue crescent) in the ER lumen.

Article Snippet: For functional analysis, Orai1 encoding sequence was also subcloned into pCI-neo (Promega, Madison, WI), and mouse STIM1 encoding sequence was subcloned from pApuro-FLAG-tagged mouse STIM1 ( 31 ) into pCI-neo.

Techniques: Glycoproteomics, Sequencing, Binding Assay, FLAG-tag, Sterility